BC 367 Experiment 4 Kinetic Properties of Acid Phosphatase

and deoxynucleotides), as allosteric effectors of certain enzymes (e.g., fructose-1-6- bisphosphate), and as ... Acid phosphatases have optimal activi...

156 downloads 310 Views 233KB Size

Recommend Documents

Jul 6, 1983 - Acid phosphatase from yeast Saccharomyces cere- visiae was purified, and its .... NaOAc buffer, pH 4.6, and absorbance at 280 nm was ...

KEY WORDS: 3D reconstruction, Close range Photogrammetry, Structured light, Digital camera image, Integration. ABSTRACT: .... camera imaging. Structured light has been applied to obtain the fine 3D model of all the object parts except the sphere. It

a radioimmunoassay technique. The study is thus designed to elucidate the precise role of immunologically measured. PSAP in diseases of the prostate gland. ... was obtained by standard procedures. PSAP was iodinated by a commercial modification of th

Jan 22, 2013 - Funding: AM is supported by a post-doctoral fellowship of the Department of Biotechnology (DBT-PDF), SKS is the recipient of senior research fellowship from the Council of ..... Fluorescence-derived thermodynamic parameters obtained fr

Feb 20, 1987 - are catabolized through analogous mechanisms for the first three steps: transamination, oxidative decarboxylation of the branched-chain a-keto ...

Apr 13, 2004 - mechanisms play a particularly important role in the intracellular destruction of phagocytosed microorganisms. This system consists of active oxygen derivatives: myeloperoxidase, as well as oxidants containing chlorine and bromine, e.g

at approx. 0-07M concentration. The buffers were prepared according to published directions as follows: glycine-NaOH, Sorensen (1912); veronal-HCl, ... followed by 3 ml. ofthe Nessler reagent, added rapidly from a wide-bore pipette. The flask .... A

acid phosphatase showed catalytic features quite different from those of the free enzyme. ..... Journal of Molecular Catalysis A: Chemical 115, 305±316.

Jul 26, 2013 - Abstract. Tuberculosis (TB) is responsible for nearly 1.4 million deaths globally every year and continues to remain a serious threat to human health. The problem is further complicated by the growing incidence of multidrug-resistant T

sediment when mitochondria are prepared according to Hogeboom, Schneider, ... in 30 per cent sucrose solution and the granules spun down again by ... N was determined according to the micro-Kjeldahl and Nessler methods, and P.

Mar 13, 1980 - &O. Yield c#. 100. 0.6. 0.4. 0.12. 0.1 1. " eIF-2 phosphatase activity could not be detected in either the pH. 5 supernatant or precipitate following the ... 7). Kinetic Properties-The activity of purified eIF-2 phospha- tase is optimu

It is the polar sites that are responsible for adsorbing solutes as they pass by. Depending on the polarity of the solute and adsorbent, some solutes are adsorbed ...

PPP4 exists as high molecular mass complexes of 450-600. kDa, and two putative regulatory subunits have been identified,. R1 (Kloeker and Wadzinski, 1999) and R2 (Hastie et al., 2000). However, the subunit composition of higher molecular mass complex

GSH, mesna or WR-1065 and A. 4OOH-CP or acrolein. Equation 1 is the ionization of the thiol, characterized by its pKa. If the kinetics to reach equilibrium for eq. 1 are rapid, the concentration of RS is propor- tional to the concentration of RSH, an

Mar 5, 2008 - generates an alternative 59UTR, is used by the hepatocytes and renal tubule cells, which are the other major sites of TRAP .... (D) Expression of monomeric TRAP in adipose tissue (T) and isolated adipocytes (Ad) from five subjects (Pat

concentration of the electrolyte. In practice, however, the molar conductivity is found to vary with the concentration. (see Fig. 1). One reason for this variation is that the number of ions in the solution might not be proportional to the concentrat

with the results obtained from SDS-PAGE (Fig. 2, lanes 4 and 5). Similar enzyme stabilization was achieved with other ionic and non-ionic detergents at low concentrations (results not shown). The concentration range of the detergents tested covered f

Mar 12, 2018 - In vitro enzyme assays indicated that GmPAP14 had a relatively high ... of inorganic phosphate (Pi), is much lower than that needed for plant growth. ..... with 0.5 mL of malachite green reagent, then measured at 650 nm.

acid phosphatase activity in Gaucher's disease serum. Maximum activity was observed at pH 5.5 to 6.0 in 0.1 mol/L sodium citrate buffer. In exploring the effect of various compounds added to the assay medium, we noted that very high concen- trations

transfer of HHD immune lymphocytes to nude mice bearing human tumors. To confirm the existence of peptide-specific. CTL precursors in human, lymphocytes from healthy and prostate cancer individuals were stimulated in vitro in the presence of these pe

Feb 18, 2010 - core protein fused to a C-terminal 6xHis tag was prepared as previously described (19). The unlabelled and the ... surements. All fitting procedures were carried out with. OriginTM 7.5 software based on nonlinear, ...... Viral Hepatiti

DPG, 2,3-diphosphoglycerate;. PTS, S-hy- droxy-1,3,6-pyrenetrisulfonate. Rate of combination of deoxyhemoglobins with carbon monoxide. The rates are calculated from the times of half-completion of the reaction, as though the reactions were second ord

Two inhibitors of erythrocyte glucose trans- port, phloretin and diethylstilbestrol, were reported not to inhibit the reconstituted transport (3), so this matter was reinvestigated. In order to determine the orientation of the transporter in the reco

OMP decarboxylase was also prepared from yeast by a method which avoided the autolysis step and which included the use of the protease inhibitor, PMSF (19, ...