BC 367 Experiment 4 Kinetic Properties of Acid Phosphatase

and deoxynucleotides), as allosteric effectors of certain enzymes (e.g., fructose-1-6- bisphosphate), and as ... Acid phosphatases have optimal activi...

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acid phosphatase to hydrolyze the various phosphate .... higher concentrations i.e. above I mM inhibited the .... Inhibition of enzyme by higher concentration of.

in the specific analysis of prostatic acid phosphatases. ... Blood. Research. Laboratory,. New. England. Medical. Center. Hospital,. Boston,. Mass.; ... this report, and its unique biochemical .... 80. Table 2. Kinetic Properties of Purified lsoenzym

Jul 6, 1983 - Acid phosphatase from yeast Saccharomyces cere- visiae was purified, and its .... NaOAc buffer, pH 4.6, and absorbance at 280 nm was ...

that were not as water soluble as 200 mmol/L were tested at the limit of their solubility. The enzymatic hydrolysis ..... 1,5-Hexanediol. 400. 151.8. 667.1. 145.1.

correlation exists between plant intracellular and secreted AP (IAP and SAP, ...... From Phytohormones to Genome Reorganization (Lerner, H. R. ed) pp.

KEY WORDS: 3D reconstruction, Close range Photogrammetry, Structured light, Digital camera image, Integration. ABSTRACT: .... camera imaging. Structured light has been applied to obtain the fine 3D model of all the object parts except the sphere. It

tions of nonspecific acid phosphatase described in this paper have activities of ... p-nitrophenyl phosphate were obtained from the Sigma Chemical. Company.

from Armour and Company. p-Nitrophenyl phosphate was ob- tained from the ... 3 A 0.02-ml aliquot of the reaction mixture was removed and added to 0.28 ml of ...

Sep 10, 2013 - known that the secretion of specialized exosomes (prostasomes) is ... an increased level of prostasomes (exosomes) has been detected in.

solution,. pH 4.8). This solution con- taiiis. 21 gm. citric acid and about 200 ml. 1 N sodium ... Chemical. Co.,. Iltem 104-9) is carefully analyzed in replicate by a reliable ... requil'ed to accommodate flow system changes is avoided. A plot relat

At temperatures between 45 and 50 C, staphylococcal acid phosphatase purified. 44-fold ... the synthetic substrate, p-nitrophenyl phosphate, was 19.5 Kcal/mole. Km for the .... A value of100 was arbitrarily assigned ..... LITERATURE CITED. 1.

incubated in the presence of a phosphatase inhibitor, sodium orthovanadate, LPA accumulated ... phosphate, a glycerol, and a fatty acid [1,2]. In spite of its.

a radioimmunoassay technique. The study is thus designed to elucidate the precise role of immunologically measured. PSAP in diseases of the prostate gland. ... was obtained by standard procedures. PSAP was iodinated by a commercial modification of th

The immunochemical methods have increased clinical sensitivity and spec ificity in detecting curable occult or confined disease, but not significantly so as to ...

was obtained in immature cells, which might include promyelocytes, myelocytes, and metamyelocytes. Mature neutrophils were stained moderately, and.

Feb 20, 1987 - are catabolized through analogous mechanisms for the first three steps: transamination, oxidative decarboxylation of the branched-chain a-keto ...

Jan 22, 2013 - Funding: AM is supported by a post-doctoral fellowship of the Department of Biotechnology (DBT-PDF), SKS is the recipient of senior research fellowship from the Council of ..... Fluorescence-derived thermodynamic parameters obtained fr

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Apr 13, 2004 - mechanisms play a particularly important role in the intracellular destruction of phagocytosed microorganisms. This system consists of active oxygen derivatives: myeloperoxidase, as well as oxidants containing chlorine and bromine, e.g

The activities of uteroferrin, measured as acid phosphatase (AP), and an .... incubated with 0-5 ml substrate in 0-05 M-phosphate buffer, pH 7-4, for 60 min .... the proteinase inhibitor, aprotinin, did not inhibit blastocyst-induced depletion of the

It was identical to the M. morganii PhoC acid phosphatase gene. Sequential in vitro ... cess is simpler, because the enzymatic reaction can be carried out in the same ... Kinetic constants for inosine in the transphosphorylation reaction were .... se

the form of the ureides allantoin and allantoic acid, which are derived from de novo-synthesized purines (Schubert,. 1981, 1986; Atkins et al., 1982; Christensen ...

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Wheat Germ Acid Phosphatase Catalyzed Hydrolysis of Para-Nitrophenyl ... analyzed and the kinetics of both an inhibited and an uninhibited reaction ... The citrate buffer used in the experiment .... range of the optimum enzyme activity being.